4

MicroSolv manufactures HPLC columns, autosampler vials for HPLC & GC, syringe filters, fittings and accessories and small bore tubing. CE, TLC, Flash Chromatography and OPLC are also available. Separation sciences leaders.

Since 1992
732.380.8900
Search
 
 

 

Cogent Diamond Hydride

Click here to view all  Applications
More Information

Amino Acid Separation without Derivatizing


Chart
This figure shows the extracted ion chromatogram (EIC) of a nineteen amino acid mixture under gradient conditions.

Click on Chromatogram to enlarge


Method Conditions and Sample Preparation Protocol

From our experience with amino acid separations in the ANP mode of separation, samples should be prepared as follows:
  1. Stock sample solution (if used) should be prepared in 100% water with 0.1& formic acid (or acetic acid: depends which acid is used in your mobile phase).
  2. Samples used for LC-MS should be diluted using 60% acetonitrile + 0.1%formic (or acetic acid) /40% DI water +0.1% formic (or acetic);
If the stock sample solution is not used sample should be prepared as in point 2 above.

Method Run Conditions:
Column: 2.1 x 150 mm Cogent Diamond Hydride™
Solvent A: DI water + 0.1% formic (or acetic) acid
Solvent B: Acetonitrile + 0.1% formic (or acetic acid)
Flow rate: 0.4 mL/min
Gradient conditions:
     0 - 5 min start at 100%B
     5 - 6 min to 90% B
     6 - 7 min hold 90% B
     7 - 9 min to 70%B
     9 - 10 min hold 70%B
     10- 18 min hold 100%B

     2 min hold 100%B (for equilibration)