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Cogent UDC Cholesterol


This is 6 Chromatograms, not 1.

Click on picture to enlarge


Reproducibility:
Cogent Silica-C™ Columns


Reproducibility


The Chromatogram shown here is actually six chromatograms laid over each other. This clearly shows how reproducible the method and column are from run to run.

Method Conditions

Column Cogent Silica-C™, 4µm, 100A
Catalog No. 40000-75P
Dimensions 4.6 x 75mm
Solvents A: DI water + 0.1 % formic acid
B: acetonitrile + 0.1% formic acid
Peaks 1. L-(+)-alpha-phenylglycine
2. L-phenylalanine
Mobile Phase 80%B/20%A
Flow rate 1.0 mL/minute (t0 = 0.85 min)
Injection Volume  2 µL
Sample Matrix 0.3 mg/mL of each sample dissolved in 50% acetonitrile/50% DI water + 0.5% formic acid.
Detection 254 nm UV