What is RSA-Pro X™ Treated Glass?

RSA-Pro X ™ Vials & Inserts Ordering Information
RSA ™ Vials & Inserts Ordering Information

New Hydrophobic Glass, Autosampler Vials & Inserts with Extreme Hydrolytic Stability.

Reduced Surface Activity (RSA™) Glass is very successful in eliminating the adsorption of basic, polar compounds that typically interact with silanols on the glass surface. This interaction can result in erratic, incorrect quantitation and/or the failure to identify an important impurity or marker. For small molecule, basic compound analytes with pKa ‘s >7 ,  consider using non-surface treated, RSA™ vials to avoid interaction issues and achieve vial to vial, lot to lot consistency. 

"RSA™ vials are made with a proprietary glass manufacturing process that does not create surface silanols (OH groups) and virtually eliminates surface metals. Also, there is no residual microgram amounts of powders or grease found in other borosilicate glass vials that occur during the conversion of glass tubing into a vial. The RSA™ surface is considered pristine and clear of any contaminants. Click HERE for more information."

The RSA-Pro X™  Technology was developed for neutral and acidic compounds that are "sticky" with glass surfaces as they interact with the glass structure itself, including siloxane bridges and common surface metals. Proteins, peptides, enzymes and many other compounds fall into this category and often require a "deactivated" glass surface treatment known as silanization to be used. A gaseous silane is applied to convert the glass surface from its natural, hydrophilic state to one that’s hydrophobic. This is old technology with many pitfalls including lack of 100% coverage of the glass, delamination of the coating exposing sections of raw glass and the total loss of the coating over time in the presence of water. These factors often render silanized glass into expensive ordinary glass vials with lost samples, data corruption and work that must be repeated. For hydrophilic compounds you should consider using RSA™ Vials.

What is RSA-Pro X™ Technology? A proprietary surface treatment of RSA™ vials to a permanent hydrophobic state that won’t degrade with time or in the presence of water. This treatment renders the hydrophobic glass surface very hydrophobic and hydrolytically stable that won’t bleed or leach into your chromatograms.

How is RSA-Pro X™ Manufactured? Starting with the pristine RSA™ vials an advanced process with covalent bonding of a special compound is applied that completely covers the RSA™ surface making it fully hydrophobic.

Every Batch of RSA-Pro X™ Glass is Tested for surface tension and hydrophobicity. Certificates of Conformance for these QC tests are available and lot support for investigations are available at no charge if needed.

A Great Benefit using RSA-Pro X™ treated vials is a much improved Quantitation %RSD from run to run, day to day and vial to vial. You can expect improved data with basic, acidic or neutral compounds. Other benefits include:

1. Get Higher Recovery of your Analytes
2. Produce Higher Sensitivity
3. Improved %RSD, Batch to Batch, Vial to Vial
4. Improved Chemical Resistance Compared to Silanized Glass.

Who should use RSA-Pro X™ Vials and Inserts? If you’re analyzing hydrophobic analytes such as most proteins, peptides, enzymes and other compounds and need the best container to ensure that your chromatography is the best possible. If you’re working with unstable compounds that interact with glass or chelate with surface metals that are inherent in ordinary borosilicate glass vials or if you need to store compounds for more than 2 weeks or need to transport them.

If you have used silanized vials and inserts and have noticed problems with less than complete coverage, inconsistent results from vial to vial, loss of hydrophobicity in water after 2 weeks; consider using the new and advanced technology of RSA-Pro X™ Vial Kits or Inserts. Click HERE for Ordering Information.

 

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Benefits of Using RSA-Pro X™ Glass Autosampler Vials and Inserts: 
 

Residual contaminants used as release agents for ordinary glass autosampler vial manufacturing processes can cause the pH of water based diluents to increase by up to 1.5 pH units in the vial over a four-hour period. RSA-Pro X™ vials do not contain any of these common contaminants and gives you assurance that your chromatography will not change during this period due to invisible vial contamination. Improved  chemical resistance compared to silanized vials.

 

Prevent hydrolysis in the vial. Hydroxyl groups found on the surface of all ordinary glass autosampler vials can be very acidic causing hydrolysis of bio active compounds. The RSA-Pro X™ vial surface has virtually no silanols or hydrophilicity making them mostly inert for these types of compounds that are susceptible to acidic degradation.

 

Assure quantitation precision run to run, minimize sample adsorption. The surface ordinary glass autosampler vials will bind hydrophobic compounds. RSA-Pro X™ vials have a surface treatment that makes it hydrophobic and every batch is tested for low adsorption of these compounds. Quantitation is very precise and eliminates high RSD vial to vial and lot to lot. Prevent investigations due to glass vials retaining your samples which are especially noticeable in low sample concentrations or low dosage forms. Improved chemical resistance compared to Silanized Glass.

 

During the manufacturing process for all ordinary autosampler vials, if the process is not controlled properly, metals such as sodium accumulate on the surface of the glass. These elements create adducts that can cause issues for your LCMS data. All batches of RSA-Pro X™ vials are tested for and have extremely low metal content, making them ideal for LCMS, LCMS/MS and GC/MS.

 

Prevent spurious peaks from glass. The RSA-Pro X™ vial manufacturing process is conducted in an ultra-clean glass conversion facility without the use of any release agents, grease or glass additives. The entire process is fully automated and the vials are never touched by human hands. This provides assurance for the cleanest vials without a wash process which causes contamination of finished vials due to residue.

 

Protect your sample needle and instruments. RSA-Pro X™ vials are manufactured with the same dimensional tolerances as the AQ™ Brand (Best-in the Industry) vials to meet the most demanding needs of all autosamplers on the market. This includes bottom height control which is critical for Waters© autosamplers. Compare RSA-Pro X™ vials to the internal dimensional control of other HPLC and LCMS Certified™ products on the market.