What is RSA™ Glass?

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Say Goodbye to Inaccurate Data

Chemists often face challenges with ordinary and even “Certified” glass vials and copy cat vials due to their reactive silanol sites, which can lead to inaccurate and imprecise data. This variability can result in flawed decisions and unnecessary investigations.

RSA™ Glass Technology - Proven QED - Since 2006

Reduced Surface Activity (RSA™) Glass Technology was developed to solve these issues. RSA™ glass vials and inserts are designed for LCMS, MS, HPLC, GC, and CE applications, providing reliable and consistent results. They minimize adsorption of basic analytes, do not add metals, and maintain the pH of the diluent. Ideal for low-abundance, pH-sensitive, and basic analytes, RSA™ glass ensures vial-to-vial and lot-to-lot consistency, protecting your mass spectrometer’s ion source from adducts.

What Makes RSA™ Technology Unique?
RSA™ vials are made using a proprietary process that eliminates silanols (OH groups) on Type 33 borosilicate glass. This process also removes the problems caused by surface metals and has no residual contaminants like powders or grease. The stringent manufacturing process ensures contaminant-free packaging and thorough quality control, including tests for adsorption, metal content, and residual materials. Certificates of QC Conformance are available upon request.

Consistent Quality You Can Trust
Every batch of RSA™ glass undergoes rigorous testing for basic compound adsorption, pH stability, metal content, and standard fit and form tolerances. Certificates of Conformance and lot support for investigations are available at no charge.

The Main Benefits of RSA™ Glass

• No Contaminants: Ordinary glass vials can increase the pH of water-based diluents by up to 1.5 pH units over four hours. RSA™ vials do not contain these contaminants, ensuring stable chromatography.

• Prevent Hydrolysis: Ordinary glass vials have acidic hydroxyl groups that can hydrolyze bioactive compounds. RSA™ vials have virtually no silanols, making them inert and ideal for sensitive compounds.

• Precision and Low Adsorption: Ordinary glass vials bind basic compounds, leading to high RSD. RSA™ vials have no silanols and are tested for low adsorption, ensuring precise quantitation and consistent results

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How You Could Benefit by Using RSA™ Glass Autosampler Vials and Inserts: 
 

Other glass autosampler vials often contain residual contaminants used as release agents during manufacturing. These contaminants can cause the pH of water-based diluents to increase by up to 1.5 pH units over a four-hour period, potentially affecting your chromatography results. RSA™ vials do not have these common contaminants, ensuring that your chromatography remains stable and accurate throughout your analysis. With RSA™ vials, you can trust that your results won’t be compromised by invisible vial contamination.

 

Other glass autosampler vials have acidic hydroxyl groups on their surface, which can cause hydrolysis of bioactive compounds. This can compromise the integrity of your samples. RSA™ vials are designed with a surface that has virtually no silanols, making them mostly inert. This ensures that your sensitive compounds remain stable and unaffected by acidic functionality, providing you with reliable and accurate results.

 

Other glass autosampler vials have hydroxyl groups on their surface that bind basic compounds, leading to sample adsorption and variability. This can result in high relative standard deviation (RSD) from vial to vial and lot to lot, especially noticeable in low sample concentrations or low dosage forms. RSA™ vials are designed without silanols, ensuring minimal sample adsorption. Each batch is rigorously tested for low adsorption, providing you with precise quantitation and consistent results. By using RSA™ vials, you can prevent investigations caused by sample retention in glass vials, ensuring reliable and accurate data every time.

 

During the manufacturing process for all ordinary autosampler vials, if the process is not controlled properly, metals such as sodium accumulate on the surface of the glass. These elements create adducts that can cause issues for your LCMS data. All batches of RSA™ vials are tested for and have extremely low metal content, making them ideal for LCMS, LCMS/MS and GC/MS.

 

Prevent spurious peaks from glass. The RSA™ vial manufacturing process is conducted in an ultra-clean glass conversion facility without the use of any release agents, grease or glass additives. The entire process is fully automated and the vials are never touched by human hands. This provides assurance for the cleanest vials without a wash process which causes contamination of finished vials due to residue.

 

Protect your sample needle and instruments. RSA™ vials are manufactured with the same dimensional tolerances as the AQ™ Brand (Best-in the Industry) vials to meet the most demanding needs of all autosamplers on the market. This includes bottom height control which is critical for Waters© autosamplers. Compare RSA™ vials to the internal dimensional control of other HPLC and LCMS Certified™ products on the market.

NOTE: RSA™ glass autosampler vials and glass inserts are manufactured without coatings. This makes them an excellent choice for HPLC, LCMS, GC and GCMS applications when quantitation, reliability and accuracy are important. With standard borosilicate glass vials and inserts, sample diluents (such as water) can de-protonate the many surface hydroxyl groups, producing a negative charge on the glass wall. The “acidic” functionality of silanols has been known to cause adsorption of basic compounds, change the sample solution pH and/or hydrolyze susceptible compounds rendering them undetectable. With RSA™ Glass vials and inserts there is virtually no sample “loss to the vial” and no “pH shifting” making your analysis data more reliable.